artesunate concentrations  (MedChemExpress)

Journal: Open Life Sciences

Article Title: Artesunate inhibits hepatocellular carcinoma cell migration and invasion through OGA-mediated O -GlcNAcylation of ZEB1

doi: 10.1515/biol-2025-1109

Figure Lengend Snippet: Artesunate inhibits HCC cell viability. (a) Chemical structure of artemisinin. (b) Normal THLE3, (c) Hep3B, and (d) HCCLM3 cells were treated with 0, 25, 50, 100, and 200 μM artesunate for 48 h, and cell viability was measured using the cell counting kit-8. * P < 0.05, ** P < 0.01, and *** P < 0.001 vs the 0 μM group.

Article Snippet: Following cell attachment, they were treated with a gradient of artesunate concentrations (0, 25, 50, 100, and 200 μM; MedChemExpress, Monmouth Junction, NJ, USA; ) for 48 h. After treatment, 10 μL of cell counting kit-8 solution (Dojindo, Kumamoto, Japan) was added to each well, and the cells were incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader to assess cell viability.

Techniques: Cell Counting

Journal: Open Life Sciences

Article Title: Artesunate inhibits hepatocellular carcinoma cell migration and invasion through OGA-mediated O -GlcNAcylation of ZEB1

doi: 10.1515/biol-2025-1109

Figure Lengend Snippet: Artesunate suppresses the cell migration and invasiveness of HCC cells. Hep3B and HCCLM3 cells were treated with artemisinin, and (a) cell migration was measured using the wound healing assay; the Transwell assay was conducted to determine (b) cell invasion and (c) migration. *** P < 0.001 vs the control group.

Article Snippet: Following cell attachment, they were treated with a gradient of artesunate concentrations (0, 25, 50, 100, and 200 μM; MedChemExpress, Monmouth Junction, NJ, USA; ) for 48 h. After treatment, 10 μL of cell counting kit-8 solution (Dojindo, Kumamoto, Japan) was added to each well, and the cells were incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader to assess cell viability.

Techniques: Migration, Wound Healing Assay, Transwell Assay, Control

Journal: Open Life Sciences

Article Title: Artesunate inhibits hepatocellular carcinoma cell migration and invasion through OGA-mediated O -GlcNAcylation of ZEB1

doi: 10.1515/biol-2025-1109

Figure Lengend Snippet: Artesunate inhibits OGA-mediated O -GlcNAcylation. (a) Immunoblotting was performed to detect the total O -GlcNAcylation level and protein levels of OGT and OGA. (b) The effect of artesunate on the total O -GlcNAcylation level and protein levels of OGT and OGA was evaluated using immunoblotting. (c) BLI analysis of the binding between artesunate and OGA. (d) The 3D structure of artesunate and the OGA binding complex was acquired using molecular docking. (e) The electrostatic surface of the protein OGA. (f) The detailed binding mode of artesunate and OGA.

Article Snippet: Following cell attachment, they were treated with a gradient of artesunate concentrations (0, 25, 50, 100, and 200 μM; MedChemExpress, Monmouth Junction, NJ, USA; ) for 48 h. After treatment, 10 μL of cell counting kit-8 solution (Dojindo, Kumamoto, Japan) was added to each well, and the cells were incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader to assess cell viability.

Techniques: Western Blot, Binding Assay

Journal: Open Life Sciences

Article Title: Artesunate inhibits hepatocellular carcinoma cell migration and invasion through OGA-mediated O -GlcNAcylation of ZEB1

doi: 10.1515/biol-2025-1109

Figure Lengend Snippet: Artesunate hampers HCC cell migration and invasion via increasing OGA expression. (a) OGA mRNA expression was measured in Hep3B and HCCLM3 cells transfected with shNC and shOGA using qRT-PCR. (b) HCC cells were treated with artesunate and transfected with shOGA; cell viability was assessed using the cell counting kit-8. (c) Quantification results of the wound healing assay. (d) Represent images of wound healing and Transwell assays. (e) Cell invasion and (f) migration analyzed using the Transwell assay were quantified. *** P < 0.001 vs the shNC group. ### P < 0.001 vs the artesunate + shNC group.

Article Snippet: Following cell attachment, they were treated with a gradient of artesunate concentrations (0, 25, 50, 100, and 200 μM; MedChemExpress, Monmouth Junction, NJ, USA; ) for 48 h. After treatment, 10 μL of cell counting kit-8 solution (Dojindo, Kumamoto, Japan) was added to each well, and the cells were incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader to assess cell viability.

Techniques: Migration, Expressing, Transfection, Quantitative RT-PCR, Cell Counting, Wound Healing Assay, Transwell Assay